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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was discovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a vital function in endorsing mobile-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two more splice variants have been reported but specific nucleotide sequence details is not nevertheless accessible.
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Blocking peptides are peptides that bind specifically to the goal antibody and block antibody binding. These peptide ordinarily includes the epitope recognized by the antibody.
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This information outlines more feasible optimization of mobile and immunoassay parameters to be sure the best possible final results are...
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This information outlines even further doable optimization of mobile and immunoassay parameters to be certain the very best success are...
Antibodies sure to the blocking peptide now not bind to your epitope over the target protein. By comparing the staining through the blocked antibody versus the antibody alone, you can see which staining is precise.
Concentrate on Information and facts Cyclin E belongs into the extremely conserved cyclin household, whose users are characterized by a spectacular periodicity in protein abundance with the cell cycle. Cyclins purpose as regulators of CDK kinases. Distinctive cyclins exhibit distinct expression and degradation styles which lead to the temporal coordination of every mitotic party. Cyclin E sorts a posh with and capabilities like a regulatory subunit of CDK2, whose action is required for cell cycle G1/S transition. This protein accumulates in the G1-S section boundary which is degraded as cells progress as a result of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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